vol. 12 núm. 22 (2014)
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Browsing vol. 12 núm. 22 (2014) by Subject "B1 gene detection."
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- Optimization of Polymerase Chain Reaction for the detection of the B1 gene of T. gondii
Institución: Universidad Nacional Abierta y a Distancia
Revista: Nova
Autores: Cortés Cortés, Liliana Jazmín; Hernández Castro, Diana Carolina; Mantilla, Mónica; Medina, María Isabel; Sofía, Duque
Fecha de publicación en la Revista: 2014-12-15
Fecha de cosecha en Ciencia Nacional: 2024-04-30
Objective: This study aimed at optimizing the amplification conditions of the B1 gene (35 copies in the genome) of T. gondii in cases of possible brain toxoplasmosis. Materials and methods: DNA was extracted from the peritoneal exudate of mice inoculated with the RH strain of T. gondii. Total exudate volume was 17 mL with a concentration of 1x107 parasites/mL. PCR conditions for the B1 gene were further optimized.Results: Serial dilutions from 1x106 to 1x10-1 parasites/mL were needed for amplifying a fragment of 132 bp . This set the detection limit for 1 T. gondii tachyzoite.