Browsing by browse.metadata.journal "Nova"

Now showing 1 - 20 of 79
  • Ability of Halophile Bacteria to Capturesodium and Its Possible Application  in Bioremediation of Saline-Sodic Soil

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Sánchez Leal MSC, Ligia Consuelo; Arguello Arias, PhD, Heliodoro

    Fecha de publicación en la Revista: 2006-12-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    The inadequate agricultural practices have been the cause of the increase of saline-sodic soils in the entire planet. Its recovery has been done mainly changing sodium by another cation, generally calcium, remedying in mechanical form and using halotolerant plants. Although these practices control the problem to a certain extent, it has not been able to make an effective recovery in these soils. This work displays as an alternative, the use of bioremediation with halophiles bacteria. The objective of the investigation was to evaluate the capacity of five species of halophiles to capture in vitro sodium ions and to design a proposal for its possible application in bioremediation of sodic and saline soils. The capture of sodium was demonstrated by comparing the initial concentration of the sodium solution without inoculating and the concentration of the same one with the bacterium inoculated through the technique of spectroscopy of atomic absorption.The bacteria that demonstrated the capacity to capture sodium in vitro were: Vibrio alginolyticus, Vibrio metschnikovii, Flavimonas oryzihabitans and Agrobacterium tumefasciens. Serratia marcescens did not demonstrate capture. The verification of the sodium capture allowed as to make two proposals: to design a bioreactor with a microbial partnership that includes the bacteria that captured sodium in the experiment and native bacteria in saline and sodic soils and to use genetic engineering to implant the gene of sodium bomb of the species with better capture in existing indigenous flora in this type of grounds.
  • Actividad antimicrobiana de líquenes de la cuenca alta del rio Bogotá

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Rodríguez A., Oscar E.; Andrade B., William A.; Díaz L., Fabio E.; Moncada, Bibiana

    Fecha de publicación en la Revista: 2015-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Determinar la actividad antimicrobiana de extractos de diferentes polaridades de las especies de Everniastrum columbiense, Flavopunctelia flaventor, Parmotrema simulans, Ramalina celastri, Teloschistes exilis y Usnea sp., recolectadas en el margen occidental de la cuenca alta del Río Bogotá, a los 05° 07’ 23,4”N y 73° 39’ 10,5”W; frente a los microorganismos Staphylococcus aureus y Klebsiella pneumoniae. Materiales y métodos. Se utilizó el método de difusión en gel por perfo­ración en placa. Resultados. Los extractos de Flavopunctelia flaventor, Everniastrum columbiense, Parmotrema simulans, Usnea Sp y Ramalina celastri, presentaron actividad antibacteriana frente a Staphylococcus aureus. Y los extractos de Flavopunctelia flaventor, Usnea Sp. y Ramalina celastri, presentaron actividad antibacteriana frente a Klebsiella pneumoniae.
  • Adaptation of Bacteria to Different Concentrations of Phenol in the Laboratory: Essentials for Biodegradation Process

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Echeverri Jaramillo, Gustavo Eugenio

    Fecha de publicación en la Revista: 2011-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    It was intended to see growth of bacterial load of contaminated samples, based on adaptation to different concentrations of phenol. Were followed in the middle mineralized concentrations (200 to10000mg/L), and variations of inoculum. Demonstrated changes in bacterial growth, increased burden found in soil, with phase adjustment on the second day and growth phase on the third day, most clearly in 200 and 500mg/L, decreasing with increasing concentration. Comparing variations in the inoculums (directly adapted and pre-enrichment), the samples can be applied to direct high load (soil), the pre-enrichment is not practical for bacterial stress, can be used and adapted to 500mg/L with no problems. In ringing on agar (mineralized and nutritious), there was growth up to 1000mg/ L, making it clear toal low detailed study biochemical characterization. It is important to purify waste water from different sources to find bacteria that resist high concentrations of phenol and allow effective ness in bioprocesses, knowing preadaptation behaviors, such as study time and appropriate treatment for the preparation of inoculum.
  • Analysis of Economic and Business Factors Influencing Disruptive Innovation in Telehealth

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Jorge Millan, Jorge Millan; Yunda, PhD Leonardo; Valencia, PhD Andrés

    Fecha de publicación en la Revista: 2017-10-16

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Objective. Analysis of economic and business factors influencing disruption innovation in healthcare taking as a reference Telehealth systems and technologies. Methods. Analysis of economic and business decision factors that influence the adoption of new technologies are qualitatively analyzed using as a case example Telehealth systems. Results. Main factors for the adoption of new technologies in healthcare are identified and analyzed. Factors include new technology adoption cost, usability, perceived value, competitive systems, old systems competitivecost and performance, and type of users, between others.
  • Analysis of studies of psychopharmacers in a IPS of the department of Córdoba 2012-2020

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Hanna Lavalle, Mónica Isabel; Fuentes Fabra, Ernesto; Arrazola, Justiniano

    Fecha de publicación en la Revista: 2022-09-13

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Psychotropic drugs are drugs used in medical practice to treat mental illnesses. Objective. To carry out an análysis comparing consumption indicators in the use of psychotropic drugs in a mental healt institution in the departamento of Cordoba Colombia. Methodology. Longitudinal, retrospective-cross-sectional study, drug use study (DUS) to compare consumption indicators such as defined daily dose (DDD), number of containers, considering the diagnosis and sociodemographic variables. Results. Population of 3,089 patients, where 48% presented a diagnosis of mental and behavioral disorder due to the use of psychoactive substances, 17% presented bipolar affective disorder and 15% paranoid schizophrenia, 70% male and 30% sex feminine. The most used medications were valproic acid in 250mg capsules, lithium carbonate in 300mg tablets and sertraline in 50mg tablets; The highest consumption was obtained by antidepressants with 45.77%, followed by antipsychotics with 23.19% and finally anticonvulsants with 22.39%. Conclusion. Differences were observed in the trend of the increase in the consumption of psychotropic drugs using as consumption indicators the defined daily dose (DDD) versus the number of containers in the years of study. This research differs from the results at the national and departmental levels in the sense that depression and other mental health problems are found to be more prevalent in men
  • Analysis of the ergonomic impact associated with cargo handling in drilling equipment workers in the oil sector

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Cohen Padilla, Harold; Carrillo Landazabal, Martha; Bedoya Marrugo, Elías; Cohen Padilla, Harold; Carrillo Landazabal, Martha; Bedoya Marrugo, Elías

    Fecha de publicación en la Revista: 2020-07-09

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    The current world and the tendencies of universalization of processes that occur through globalization, the opening of markets, internationalization, competition and all the new situations that business development brings every day make processes must be more efficient and risks, with which there are risks associated with activities that involve physical exertion and therefore a possible musculoskeletal injury. Analyze the relationships identified between the negative impact tasks and the ergonomic variables associated with cargo handling, by using the REBA and RULA assessment tools so that risk factors are identified by Drilling & Well workers Services, especially wedges and pollsters of an oil company. The research is descriptive, since it represents an experimental analysis that aims to establish the initial conditions and determine the way in which a particular circumstance is manifested by identifying the characteristic features of the operation. There were activities such as lifting and positioning the wedge, moving the drill pipe, releasing or adjusting the power keys and adjusting the arms of the elevators to close and with it controlling the drill pipe, as the most important risk actions in the operations of the wedges and operators.
  • Antimicrobial Effect in Vitro of Argentineans Colombians and Cuban Propolis On Streptococcus Mutans Atcc 25175

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Figueroa, Judith; Martínez, Patricia; Moreno, Zulma

    Fecha de publicación en la Revista: 2007-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    It was evaluated the antimicrobial activity of four propolis extracts from Argentina, five from Colombia and one from Cuba, using Streptococcus mutans ATCC 25175. The samples were kept in a 96% ethylic alcohol solution, remaining a concentration of 3%. The bactericidal and bacteriostatic activity was measured by the minimun inhibitory concentration among a range of 0.02 and 15mg/ml. All of the analyzed samples showed activity against Streptococcus Mutans when they are used to concentrations 15 a 3.75 mg/ml. The propolis that presented the highest effect bactericidal was number 2 and 3 (sample from Colombia) after 48 hours of incubation.The highest effect bacteriostatic was presented by the sample 2 (propolis from Colombia) after 24 hours of incubation. 70% of the propóleo samples increased their activity after a time of incubation of 48 hours compare to the effect shown to the 24 hours. All the Colombian samples showed a superior effect, when the bacteria were exposed to the propóleo for a longer period of time; in two of these cases until of five dilutions, samples 2 and 3, this effect was presented in a moderate way, using the argentine samples 6 and 10 while the other samples (30%) remained steady.
  • Application of Indicators of Knowledge Has More Than Enough Biodiversity for Diagnose and Comparison of Biological Collections

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Vallejo, Martha Yaneth; Acosta Rueda PhD, José Alberto

    Fecha de publicación en la Revista: 2005-12-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Most biological collections from Colombian museums lack diagnosis, which prevent to anmize and to know it real state in qualitative and quantitative terms. To solve this problem it has been proposed some biodiversity indicators, which can be used on biological collections data bases, allowing museums to define it condition, priorities and to register it contribution to country biodiversity. There are basic criteria to elaborate indicators such as: scientific validation, data availability and reliability; the later including representativity, sensibility, simplicity, relevance and utility among others. They are necessary to support each indicator as part of the methodological sheet. The indicators proposed are used to compare the same biological group: (i). Between different collections; as this study case: Museo Javeriano - MUJ against Museo de Historia Natural Marina de Colombia- MHNMC and (ii). One Museum against a total reported for the country or the world.The indicators used were: representativity and complementarity, both in taxonomic as in geographic terms; danger species; taxonomy identification; data completeness, sampled intensity, and metadata index. The indicators showed the following results for the MUJ collection: registred species 30 hermatipic corals; absent species, 52 ahermatipic corals; sampling in three ecoregions in the country, temporal, 91.6% of the material collected recently, 2004; spatial distribution of the specimens collected, 6 ecoregions along the colombian Caribbean; taxonomic representativeness, 50% against MHNMC and 26% against a total documented in the Caribbean; curatory level, 94.4% material identify to species; danger species included in the collection, 17.6%; family with more repetitions, Faviidae, and most representative; Agariciidae. The use of indicators increases the collection utility, disclosure (country biodiversity) and specialization through the documented information. They allows museums to make diagnosis for each collection in order to establish management priorities and optimization of the economical resources.
  • Asociación de HSP60 de Chlamydia trachomatis y desarrollo de cáncer de ovario

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Jutinico Shubach, Adriana Paola; González Devia, Johanna Lizeth; Sánchez Mora, Ruth Mélida

    Fecha de publicación en la Revista: 2017-10-26

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Las proteínas de choque térmico (HSP) son una familia de proteínas altamente conservadas cuya expresión se regula por la exposición a condiciones de estrés. Chlamydia trachomatis (CT) es una bacteria intracelular obligada de transmisión sexual causante de infección crónica y persistente que posibilita la expresión de proteínas tipo HSP, una de estas proteínas es la HSP60 Clamidial (cHSP60) que posee una homología del 50% con respecto a la proteína HSP60 humana. Estarevisión presenta estudios immunoepidemiológicos que han demostrado una asociación entre la respuesta inmune a cHSP60 y secuelas de la infección por CT generadas por la inflamación crónica, una de estas secuelas probablemente es el desarrollo de cáncer de ovario, patología que según la OMS es uno de los cánceres ginecológicos más letales y ocupa el quinto lugar en muertes por cáncer entre las mujeres. En esta revisión se discute como la presencia de la cHSP60 ocasiona una respuesta de anticuerpos prolongada como una fuente de estimulación antigénica e inflamación crónica, ocasionando reactividad cruzada que se asocia al desarrollo de cáncer de ovario.
  • Assessing Iron Functional Status in High-Performance Sports Leagues and Skating Waterpolo Cali, Colombia

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Castillo Bohórquez MSC, Martha; Mora Bautista MSC, Ana Isabel; Aldana, Laura; Bermúdez, María Inés; Piraneque, Alejandra

    Fecha de publicación en la Revista: 2012-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    This study aimed to assess the iron functional state in a population of 43 high performance Colombian athletes belonging to Cali skating League (41.9%) and water polo (58.1%, aged 17 - 43, determining serum ferritin, soluble transferrin receptor and soluble transferrin receptor index, demonstrating the presence of subclinical iron deficiencies that lead to risks in the performance of the athlete. 55.8% of the athletes showed normal results, 41.9% had serum ferritin values below the biological reference interval and 2.3% subclinical iron deficiency grade II. The results does not show significant difference between these disciplines.
  • Bacillus sphaericus: vector control strategies that produce malaria, yellow fever, and dengue

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Gómez Romero, Sara Esmeralda; Hernández Rodríguez, Cindy Zulay; Corrales Ramírez, Lucía Constanza

    Fecha de publicación en la Revista: 2009-12-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    The increased incidence of diseases of public health such as malaria, dengue and yellow fever associated to side effects of the use of chemical pesticides and the resistance generated has prompted the search for new alternatives for the elimination of its vectors. A viable option is the entomopathogenic bacteria Bacillus sphaericus, first described in 1904 by Neide and Meyer who discovered spore-forming bacteria that exists in nature and is easily grown in vitro and in the body of the larvae.Its larvicidal activity is due to the production of a crystalline inclusion, called binary toxin A and B of 41.9 and 51.4 kDa, respectively, which, when released in the gut of susceptible insects in their larval stage and after be solubilized by the alkaline pH in the middle portion of the intestine, activate proteases causing dehydration and death of the vectors. It is considered a highly potential and effective larvicide for biological control of Anopheles spp., Aedes spp. and Culex spp. It can be produced commercially by submerged fermentation with small-scale waste protein material. This product has been used and registered in many Latin American countries, where good results were obtained after application.
  • Bacillus thuringiensis biotechnology in agriculture

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Portela-Dussán, Diana Daniela; Chaparro-Giraldo, Alejandro; López-Pazos, Silvio Alejandro

    Fecha de publicación en la Revista: 2013-12-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Bacillus thuringiensis (Bt) is a Gram-positive rod-shape bacterium that during its sporulation phase produces a parasporal inclusion formed by Cry proteins having biological activity against pest insects. Because of these proteins, Bt shows toxicity toward pest insect larvae of Lepidoptera, Co-leoptera and Diptera orders among others, besides of friendly with the environment, and for this reason Bt has a very common use for commercial products and transgenic plants with Cry protein basis in agriculture. In this review is described the most important Bt factors and their application as biotechnological tool in agrobusiness, considering Bt bioassays.
  • Bacteria, nutrition and growth: a look from chemistry

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Caycedo Lozano, Liliana; Corrales Ramírez, Lucía Constanza; Trujillo Suárez, Diana Marcela; Caycedo Lozano, Liliana; Corrales Ramírez, Lucía Constanza; Trujillo Suárez, Diana Marcela

    Fecha de publicación en la Revista: 2021-09-20

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Nutrition is a set of processes and reactions by which living beings take from the environment, in which they inhabit, the chemical substances they need to grow, multiply and make use of energy. The aforementioned substances are called nutrients and are used for two purposes: energetic when required for maintenance and biosynthetics when required for the synthesis of components (anabolism). In the first case (energetic) the bacteria are divided into lithotrophs when they make use of simple inorganic substances such as (SH2, S, NH3, NO2-, Fe, among others); and organotrophs when their requirement is for organic substances (carbohydrates, hydrocarbons, lipids, proteins and alcohols, among others). In the second case (biosynthetics), they can be differentiated into: autotrophs, when the synthesis is carried out from simple inorganic substances (CO2) and heterotrophs when their carbon source is organic, but they can also use other elements than C, which they can be captured in inorganic form. Whether autotrophic or heterotrophic, all bacteria require a series of chemical substances, which can be classified into macronutrients or micronutrients according to the amount of these required of these substances that are required.
  • Bioética de la atención de enfermería al enfermo terminal

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: León Correa, Francisco Javier

    Fecha de publicación en la Revista: 2015-05-13

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Aunque parte importante de las decisiones que han de tomar los equipos de salud en la atención a enfermos terminales corresponde al médico o al equipo médico, no es menos cierto que las consecuencias de esas decisiones afectan de manera muy clara el quehacer diario y la conciencia de las enfermeras que deben cuidar y atender a esos pacientes. Y, por otra parte, los cuidados de enfermería que deben darse a los enfermos terminales también plantean sus propias tomas de decisiones éticas que han de saber resolver-o intentar resolver- las propias enfermeras. Además, en la fase final de la vida «disminuye lentamente la función del médico y se hace más importante la de la enfermera: pasa a ser una importante fuente de información y consulta, y toma cada vez más la iniciativa
  • Biomechanical Analysis of Ongoing Digital Prosthetic Amputee above Knee

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Benavidez Noguera, Cristian Camilo; Torres Ruiz, Andrea

    Fecha de publicación en la Revista: 2009-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    This research, conducted at the BIOMED Digital Biomechanics Laboratory, shows a case study of a patient with amputation above the knee and the monocentric knee mechanism, which employs optoelectronic systems to capture the movement and force transducers plates to obtain data on kinetics and kinematics of the limb. The results were analyzed to try to recognize the major patterns, characteristics and possible improvements in gait, which could provide some basis for the future design of artificial limbs, so they are increasingly functional and similar to the pattern of normal gait.
  • Biotechnological applications of microorganisms

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Ostos Ortíz, Olga Lucía; Rosas Arango, Sonia Marcela; González Devia, Johanna Lizeth; Ostos Ortíz, Olga Lucía; Rosas Arango, Sonia Marcela; González Devia, Johanna Lizeth

    Fecha de publicación en la Revista: 2019-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    The biodiversity of microorganisms as well as the unique nature and biosynthetic capabilities in specific environmental conditions make microorganisms the likely candidates to solve problems of food shortages, pest control, biodegradation of xenobiotics, decomposition of garbage, batteries of produced waste, among others. Microorganisms offer great potential for the exploration of molecules and processes, and knowledge of non-conventional species, especially within the Archaea group, has stimulated the molecular investigation of genes of interest. These new genes can be incorporated by recombinant technology into biologically known species, such as E. coli and S. cerevisiae, for the large-scale synthesis of products. Technological microbiology has great potentials to explore and obstacles to overcome. Therefore, only research in this area is promising for scientists around the world. In this review we present the most significant applications of microorganisms in the food industry, agriculture, chemical compounds, fuels, pharmacology and materials.
  • Blattella germánica (Blattodea: Blattellidae) como potencial vector mecánico de infecciones asociadas a la atención en salud (IAAS) en un centro hospitalario de Villavicencio (Meta-Colombia)

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Jaramillo, Gloria Isabel; Pavas, Norma Cristina; Cárdenas, Juan Camilo; Gutiérrez, Paola; Oliveros, William Andrés; Pinilla, María Angélica

    Fecha de publicación en la Revista: 2016-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

      Identificar las especies de cucarachas y bacterias asociadas a su exoesqueleto en un centro hospitalario de la ciudad de Villavicencio (Meta, Colombia). Métodos. Se realizaron capturas manuales de cucarachas en cocina, urgencias, UCI intermedia, consulta externa y neonatos. Los individuos col­ectados fueron sometidos a un aislamiento primario en caldo BHI, para luego pasar a medios sólidos (sangre y MacConkey). Se realizó identificación y antibiograma por método automatizado. Resultados. Se colectaron 24 ninfas y adultos de Blattella germánica. Se aisló e identificó Klebsiella pneumoniae con sensibilidad intermedia a meropenem (CMI 4) y resistencia a cefalosporinas (cefepima y cefuroxima) (>16), Proteus vulgaris con resistencia a cefalosporinas (ceftriaxona, cefuroxima) (CMI >16), Enterobacter cloacae con resistencia a cefalosporinas (cefoxitina, cefuroxima, ceftriaxona) (CMI > 16), Enterococcus faecium, Enterococcus rafinosus, Staphylococcus xylosus y Enterococcus faecalis. El 80% de las bacterias aisla­das presentaron algún grado de resistencia a antibióticos. Conclusiones. Estos insectos podrían jugar un papel importante en la transmisión de las Infecciones asociadas a la atención en salud (IAAS). Debido a su presencia en hospitales y el reporte de IAAS y resistencia bacteriana en varios centros asistenciales en el Departamento del Meta, se hace necesario establecer la relación de estos insectos con estos eventos.
  • Caracterizacion de cafés torrefactados, provenientes del ecotopo E-220A

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Diaz, P; Riaño, C E

    Fecha de publicación en la Revista: 2017-10-26

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Antecedente. Los ecotopos son entornos con condiciones edafológicas, acuíferas, y ambientales bien definidas, en las cuales se producen granos con características de calidad que cautivan mercados locales e internacionales. Objetivo. Correlacionar fisicoquímicamente café crudo y torrefactado proveniente de 2 zonas del Ecotopo_E-220A. Materiales y método. La investigación, se desarrolló en cuatro etapas con cafés variedad Colombia de tipo supremo y extra especial, provenientes del departamento de Nariño de los municipios de La Unión y de Buesaco, siguiendo un diseño experimental de bloques al azar con análisis multivariado, complementado con la técnica de componentes principales. Resultados. Se correlacionaron las zonas de estudio con las características intrínsecas del café crudo_ mayor altura, color verde intenso, granos grandes más uniformes y de mayor acidez titulable. Y a menor altura mayor contenido de lípidos. Los cafés de la zona 2_ mayor altura_ tienen menor acidez titulable, menor contenido de lípidos, presentan la mayor pérdida de estos durante la torrefacción y genera cafés torrefactados de color entre medio y medio ligero. La zona 1 por el mayor contenido de lípidos genera cafés más aromáticos.Igualmente, con la metodología de componentes principales se determinó que la de pérdida de peso en café tostado, los carbohidratos y la densidad aparente explican el primer componente en 0.846, 0.745, 0.845 respectivamente y el segundo componente agrupa al contenido de lípidos en 0.801 y el contenido de proteína cruda en 0.620.
  • Cell Cycle by Gram Stain and Modified Fluorescence in Bacteria Resembling Morfotintorial Neisseria Gonorrhoeae Isolated from Anal and Urethral Samples Cell

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Flores Fernández, Evelin Margarita; Álvarado Ysasis, Luzmila Sofía

    Fecha de publicación en la Revista: 2009-06-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Se evaluó el ciclo celular por Gram y tinción de fluorescencia modificada en bacterias aisladas de muestras perianales y uretrales, con observación en frotis de diplococcus Gram negativos o diplococcus anaranjados fluorescentes y cultivo negativo para Neisseria gonorrhoeae, de pacientes que acudieron a la consulta ITS-SIDA del ambulatorio “Dr. Arquímedes Fuentes Serrano”, Cumaná, Venezuela, con el objetivo de mostrar que en las bacterias aisladas, las características morfotintoriales por Gram y fluorescencia semejantes a diplococos, están asociadas al ciclo celular. Se hizo estudio microscópico del ciclo celular, a partir de cultivos sincrónicos a intervalos de tiempo de 5 minutos por 2 horas, tomando alícuotas, fijando en etanol, realizando extendidos y coloreando con Gram y fluorescencia.Se identificó Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Citrobacter koseri, Staphylococcus epidermidis, S. saprophyticus, Streptococcus b-hemolítico grupo no A-no B y Enterococcus faecalis. Los cocos Gram positivos fueron los que expresaron características morfotintoriales de diplococcusGram negativos. Streptococcus b-hemolítico de 0-15 min, E. faecalis de 60-80 min, S. saprophyticus a los 10y 35, S. epidermidis a los 0,15, 20, 35, 40, 70, 90 y 95 min. Los Staphylococcus por fluorescencia, ademásde visualizarse como diplococcus anaranjados fluorescentes, se observaron verdes y amarillos. El colorvarío cíclicamente, iniciando como amarillo, pasando por anaranjado y luego verde. S. saprophyticus de40-120 min se obsevaron amarillos fluorescentes. S. epidermidis aún a los 50, 60 y 65 min se visualizaroncomo diplococcus anaranjados fluorescentes. Se concluye que los cocos Gram positivos presentan fase dediplococcus Gram negativos y anaranjados fluorescentes en su ciclo celular.
  • Characterization of molecular alterations of hemoglobin in Afro-Colombians

    Institución: Universidad Nacional Abierta y a Distancia

    Revista: Nova

    Autores: Castillo, Martha; Oliveros, Ana Lucia

    Fecha de publicación en la Revista: 2014-12-15

    Fecha de cosecha en Ciencia Nacional: 2024-04-30

    Objective: Characterize molecular alterations of hemoglobin in Afro-Colombian subjects, ranging in age from 18 to 50 years. Analyses were conducted via complete blood count, peripheral blood smear, C-Reactive Protein, serum Ferritin, Soluble Transferrin Receptor (sTfR) and electrophoresis. Materials and Methods: A total of 56 subjects were analyzed, 10 men and 46 women. Results: Subjects (from the Aguablanca District in the City of Cali) showed alterations in the hemoglobin chain associated with Hemoglobin S, Hemoglobin C and possible thalassemias due to Fetal Hemoglobin and augmented Hemoglobin A2. Additionally, a small incidence of subclinical deficiencies of iron at different stages was also detected.