Most biological collections from Colombian museums lack diagnosis, which prevent to anmize and to know it real state in qualitative and quantitative terms. To solve this problem it has been proposed some biodiversity indicators, which can be used on biological collections data bases, allowing museums to define it condition, priorities and to register it contribution to country biodiversity. There are basic criteria to elaborate indicators such as: scientific validation, data availability and reliability; the later including representativity, sensibility, simplicity, relevance and utility among others. They are necessary to support each indicator as part of the methodological sheet. The indicators proposed are used to compare the same biological group: (i). Between different collections; as this study case: Museo Javeriano - MUJ against Museo de Historia Natural Marina de Colombia- MHNMC and (ii). One Museum against a total reported for the country or the world.The indicators used were: representativity and complementarity, both in taxonomic as in geographic terms; danger species; taxonomy identification; data completeness, sampled intensity, and metadata index. The indicators showed the following results for the MUJ collection: registred species 30 hermatipic corals; absent species, 52 ahermatipic corals; sampling in three ecoregions in the country, temporal, 91.6% of the material collected recently, 2004; spatial distribution of the specimens collected, 6 ecoregions along the colombian Caribbean; taxonomic representativeness, 50% against MHNMC and 26% against a total documented in the Caribbean; curatory level, 94.4% material identify to species; danger species included in the collection, 17.6%; family with more repetitions, Faviidae, and most representative; Agariciidae. The use of indicators increases the collection utility, disclosure (country biodiversity) and specialization through the documented information. They allows museums to make diagnosis for each collection in order to establish management priorities and optimization of the economical resources.

Coral Collection Metadata (Scleractinia) from the Museo de Historia Natural Lorenzo Uribe S.J.: The necessity to notify scientist about the biological collections is a daily need, particularly when there are few of them in a country for a particular taxa, or when it.s difficult the access to them. Although the collection data bases compile information in systematic form, they are not design to divulgate the information in a universal language. To solve this problem, tools such as the metadata were created, data over data, they use the collection data base to summary the information contained, their quality; where they were collected, and state; allowing information access in a universal language. The steps we followed to generate the metadata from the coral collection referenced to the Museo de Historia Natural Lorenzo Uribe S.J. (MUJ) were: revision, digitations, organization, depuration, and standardization at taxonomic and geographic level for each biological specimen, using an international standard.The metadata allow us to know the Scleractinian genera list (MUJ), and to condense information on: 1. author and Scleractinian list citations; 2. Geographic sampling cover; 3. Conditions to access and use; and 4. temporal information and hipervinculum; within other aspects. In the same way, we applied the biological index of metadata documentation (76.6%) to quantify inter-operability with other metadata and it divulgation. Coral MUJ metadata is effective because it facilitate search of coral information in Colombia, in an efficient, specific, and complete way, this is because there are only two collections referenced. In addition it will allow us to show our biodiversity and to compare and interchange the information internationally.

Present anaerobic bacteria in gingival sulcus of patients with fixed partial dentures Recently, the biological aspects of fixed reconstructions have been given attention because dental materials have great capacity to accumulate and retain plaque. The microflora at both tooth and fixed partial prostheses can be identified as biofilms, which are composed of microcolonies of bacterial cells distributed in a shaped matrix. The purpose of this study was to isolate anaerobic bacteria in gingival sulcus from patients during fixed partial denture treatment. 45 samples were analyzed. 30 patients with fixed partial dentures treatment and 15 subjects without partial denture treatment were enrolled in the study. Microbiologic samples were obtained by inserting paper points into the gingival sulcus.Samples were cultured for anaerobic bacteria on blood agar. The plates were placed into an anaerobic chamber. Later, aerotolerance tests were performed for each different colony. RapID TM ANA II System Remel was used for identification of anaerobes. A total of 12 anaerobic bacteria were observed: 8.33 % gram–negative bacilli, 58.33 % gram-positive bacilli, 0 % Gram-negative cocci, and 33.33 % gram-positive cocci. In the control group none anaerobic bacteria was isolated. Only facultative gram-positive cocci were observed (100%). The results of this study have confirmed that the presence of fixed partial dentures resulted in a change in the composition of the anaerobic microflora of the gingival sulcus, compatible with periodontal disease.