Free-living amoebas are microorganisms present in different environments such as water or soil. They are maintained in ecosystems and can cause serious diseases due to their ability to carry intracellular bacteria to their hosts that are resistant to phagocytosis. Afterwards they invade causing bacterial disease, which makes these microorganisms an important vehicle of transmission of various pathologies of interest in public health. For this investigation, the fecal samples with signs of diarrhea were collected from an Amazona aestiva xanthopteryx (speaking parrot from chaco) and were processed by direct parasitological and spontaneous sedimentation methods. Swabs from the sewage area were sent to bacteriology and then direct seeding was carried out. With the help of the analysis of the samples, the presence of Entamoeba coli was identified as an unpublished record for the species. This study contributed to the knowledge of this problem, especially with the presence of Escherichia coli in the clinical picture.