Leptospirosis is a disease that affects both animals and humans; produces economic losses, and the human produce inespecific signs of bleeding to kidney damage. This study investigated the presence of antibodies of serovars L. gryppotiphosa, L. icterohaemorragiae L. pomona, L. bratislava, L. canicola and L. hardjo, in wild animals captured through type Sherman and Tomahawk traps over 8 months, covering an area of 107,800 m2, using the methodology of lineal fixed transects and the use of food as bait attractive to these species. The 23 individuals caught of 7 different spe-cies were sampled for obtaining serum and carry out laboratory testing using the technique of Microaglutination Test (MAT). Of the 23 individuals captured, we found evidence of the presence of Leptospira in 16 of them. In terms of punctual prevalence, this is equivalent to 69,56% (16/23). All serovars showed positive for the majority of the target population: to serovar L. hardjo 2 were positive, to L. pomona 4, to L. Icterohaemorragiae 12, to L. bratislava 3, to L. canicola 5, and to L. grippotyphosa 1, finding a prevalence for serological serovar L. icterohaemorragiae. (44,44%).

The aim of the environmental enrichment is to increase the captive animal welfare, by providing them with stimulus that motivate the fulfilment in the specie natural behaviour. An environmental enrichment program was tested on the jaguar´s (Panthera onca) and cougar´s (Puma concolor) population at the Zoológico Jaime Duque, the objective was to confirm if the welfare really increased, Faecal cortisol was monitored by ELISA test, for this, validating the technique for each specie was needed. The carried out test turned out to be valid for both species; however, the cortisol levels are influenced by many multiples variables, and it was not evident the reduction of the levels with environmental enrichment.