This paper describes the standardization of a reverse transcription (RT) polymerize chain reaction (PCR) in order to be used as a tool to detect the bovine viral diarrhea virus (BVDV). The primers employed amplified a sector of 280 bp that is in the 5’ UTR region of the viral genome. The VDVB NADL and Osloss strainswere used as positive controls for the standardization process.. In order to evaluate crossreaction a strain of border disease (BD) was utilized. The cDNA was obtained using the random primers method. The RT-PCR detected cytophatic as well as non-cytophatic byotipes of BVDV. The standardized protocol showed a good performance in vitro and it is the bases for evaluation and validation essays of this diagnostic test.