The aim was to compare the vegetation cover 2015 year with the agricultural frontier presented in the last national agricultural census in the Teusacá River sub-basin. Through a multitemporal analysis, the dynamics of agricultural areas, forests and urbanized areas in the Teusacá River sub-basin were determined. It was found that the area of forest and natural herbaceous vegetation was 14,143.29 hectares in 2015, occupying 39% in the study area. A 1% increase in forest area was observed with respect to the 1997 record; mainly detected on the villages: La Diana, Aposentos, Meusa and La Violeta in the Sopó municipality, Salitre and Santa Isabel in the Guasca municipality and San Cayetano, Frailejonal and El Volcán in La Calera municipality. Also, there was an increase in the vegetation cover in the southeast sector of the Mercenario and San Gabriel villages of the La Calera municipality and in the Salitre village between Guasca and Sopo. However, the quality of the Teusacá River water resource is being affected mainly in the Sopo sector by uncontrolled industrial and domestic dumping. It is suggested to carry out educational campaigns so that the inhabitants understand the importance of conserving, reforesting, keeping the water in good conditions so that the sub-basin maintains a sustainable ecological balance that guarantees the environmental services that it offers. Key words: Drainage area; vegetation maps; ecological balance; environmental conservation