An adequate implementation of biosafety protocols requires adequate knowledge and attitudes in favor of biosafety, on the part of the workers of the organizations. Therefore, given the health emergency situation due to COVID-19, it is important to adopt biosafety measures in all settings, which allow the appropriate deployment of biosafety protocols. This research sought to describe the knowledge and attitudes about biosafety and biological risk factor during the COVID-19 pandemic in a group of school teachers in the Bucaramanga metropolitan area. The information was collected through a virtual questionnaire and the sample was non-probabilistic. As main findings, it was found that the majority of teachers, with a result of 91%, totally or partially agree that biosafety protocols help prevent contagion by coronavirus and that biosafety protocols are implemented in the workplace. adequately with 88%.