El presente estudio pretende valorar el comportamiento de la adición del reactivo bloqueador de anticuerpos heterófilos (HBR) en la cuantificación de Troponina I cardíaca (TnIc) en pacientes con insuficiencia renal crónica terminal (IRCt) sometidos a tratamiento de hemodiálisis.Acada uno de los70 pacientes con IRCt en tratamiento de hemodiálisis en el Hospital Universitario Clínica San Rafael de Bogotá, se le tomó una muestra de suero y una de plasma heparinizado en la prediálisis, en las cuales se cuantificaron las concentraciones de TnIc con y sin adición de HBR, mediante el enzimoinmunoensayo Axsym. Se utilizó la prueba estadística de Wilcoxon. Los valores de TnIc en plasmas y sueros con y sin la adición de HBR no mostraron diferencias significativas (0.113 y 0.666 respectivamente), tanto en el total de los pacientes como en aquellos con enfermedad cardíaca crónica. El no haber encontrado diferencias en los valores de TnIc con y sin HBR nos lleva a pensar en la ausencia de anticuerpos heterófilos o en que el reactivo bloqueador HBR no tiene acción en esta población de pacientes.

Absence of interferences for heterophilic antibodies in the quantification de cardiac Troponin I, in patients with terminal chronic renal inadequacy in hemodialysis treatment. The present study seeks to value the behavior of the addition of the blocking reagent of heterophilic antibodies (HBR), in the cardiac Troponin I quantification in patient with terminal chronicle renal inadequacy subjected to hemodialysis treatment. To each one of 70 patients with IRCt in hemodialysis treatment in the San Rafael University Hospital of Bogotá, they were taken a sample of serum and one of plasm heparanized in the pre dialysis, in which the TnIc concentrations were quantified with and without HBR addition, by means of the enzimoinmunoassay Axsym. The statistical test of Wilcoxon was used. The TnIc values in plasms and serums with and without the HBR addition didn't show significant differences (0.113 and 0.666 respectively), so much in the total of the patients as in those with chronic cardiac illness. Not having found differences in the TnIc values with and without HBR it takes us to think of the absence of heterophilic antibodies or in that the blocking reagent HBR doesn't have action in this population of patients.

Introducción: El Virus del Papiloma Humano (VPH) es relevante en el desarrollo del cáncer de cérvix, situación que ha conducido a la tamización con citología cérvico-uterina y prueba molecular ADN-VPH que permiten la detección temprana de mujeres en riesgo de desarrollar esta patología. Aunque han disminuido la incidencia y mortalidad, no han logrado erradicar la enfermedad. Objetivo: Identificación del VPH en diferentes muestras de pacientes con diagnóstico de lesiones preneoplásicas y cáncer cérvico-uterino.  Metodología: Se incluyeron pacientes con lesiones de alto grado (LEI-AG) y/o carcinoma en citología cervicouterina y/o biopsia. Al total se les tomó muestra de sangre, se evaluó la disponibilidad del tejido parafinado (FFPE) y se realizó genotipificación por PCR para VPH. Resultados:  27%(3) LEI-AG estuvieron entre los 30-39 años y 9%(1) en menores de 29;  50%(6) fueron positivas para VPH-AR diferentes al 16-18 en citología en base líquida (CBL) y 16% (1) negativa. Se obtuvo DNA del 100% de los FFPE, con positividad en el 67% (6), 2 (33%) para VPH-16 y 4 (67%) para otros ( 56,58 y 68). 1(11%) presentó coinfección con VPH-56 y 58.  67% (4) mostraron concordancia entre la CBL y el FFPE; 1 fue negativa en CBL y positiva para VPH-56 en FFPE y 1 fue positiva para VPH-18 en CBL y negativa en FFPE. No se obtuvo positividad para VPH en las muestras de plasma analizadas. Conclusión: La relación VPH y cáncer cérvico-uterino requieren desarrollar estudios regionales que permitan su entendimiento y reevaluar las políticas actualmente implementadas en el país.

Introduction. The Human Papilloma Virus (HPV) is relevant in the development of cervical cancer, a situation that has led to screening with cervical-uterine cytology and DNAHPV molecular test that allows the early detection of a risk of developing this pathology in women. Although the incidence and mortality have decreased, science could not eradicate this disease. Objective. Identified HPV in different samples of patients diagnosed with preneoplastic lesions and cervical-uterine cancer. Methodology. Patients with high-grade lesions (LEI-AG) and/or carcinoma on cervical cytology and/or biopsy were included. A blood sample was taken from all of them, the availability of paraffin tissue (FFPE) was evaluated, and PCR genotyping for HPV was performed. Results. 27% (3) LEI-AG were between 30-39 years old and 9% (1) were under 29; 50% (6) were positive for HR-HPV other than 16-18 in liquid-based cytology (CBL) and 16% (1) negative. DNA was obtained from 100% of the FFPE, with positivity in 67% (6), 2 (33%) for HPV-16 and 4 (67%) for others (56, 58 and 68). 1 (11%) presented coinfection with HPV-56 and 58. 67% (4) showed concordance between CBL and FFPE; 1 was CBL negative and HPV-56 positive on FFPE and 1 was CBL HPV-18 positive and FFPE negative. No HPV positivity was obtained in the plasma samples tested. Conclusion. The relationship HPV and cervical-uterine cancer requires to work out in regional studies that allow to understand and to reassess the policies that are currently implemented in the country.

Obesity and its complications are one of the major pandemics at the global level, especially type 2 diabetes that is associated with insulin resistance. The purpose of this paper is to describe the relationship between obesity and other cardiovascular risk factors of university students in downtown Bogota. After obtaining an informed consent, we proceeded to the measurement of the following body parts: triceps skinfold and suprascapular through adipometer, waist circumference, body mass index, lean mass, fat mass and fat glucose, total percentage among others. Blood samples were also taken to determine blood cholesterol, triglycerides, and lipoproteins, whose relationship allows the calculation of the atherogenic index.From a final sample of 76 students, 15.5 % was overweight; 76.5 % was considered within normal ranges; and the 8.0 % was underweight. In regard to the data by sex, it was found that a 5.5 % of men and 19.0 % of women were overweight and 5.5 % of men and 8.6 % of the women had low weight, there were no obese people in any case. The waist perimeter varied between 68 and 97 cm in men and between 61 and 92 cm in women, finally the fat percentage was between 27.6 and 30.7 in men and from 11.0 to 36.9 in women. Taking into account the results obtained, it is important to promote in our population a culture of healthy habits, characterized by a healthy diet and increased physical activity; it is also recommended, especially during the first years of life, as it is in this stage where the majority of these habits are acquired and will last for life.